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. 1999 Oct 12;96(21):12010–12015. doi: 10.1073/pnas.96.21.12010

Figure 1.

Figure 1

Effects of POVPC, PGPC, and OxPAPC on leukocyte binding and adhesion molecule expression. OxPAPC, POVPC, and PGPC, which had been resuspended in chloroform, were dried under nitrogen and resuspended in tissue culture medium. (A) HAEC were treated with POVPC or PGPC at the indicated concentrations in μg/ml for 4 h at 37°C. Monocyte and polymorphonuclear neutrophil (PMN) binding then was performed in 48-well plates, and cell surface ELISAs were used for determination of VCAM-1, E-selectin, and CS-1 expression in 96-well plates. (B) HAEC were treated for 4 h with 100 μg/ml of OxPAPC, and experiments were performed as in A. Data are expressed as % of control (mean ± SD, n = 6 wells or fields/data point), where control represents cells treated with medium only. ★, P < 0.001 as calculated by one-way ANOVA. LPS (2 ng/ml) was used as positive control. Each experiment is representative of four independent studies.