Figure 3.

Inhibitory action of POVPC and OxPAPC on neutrophil binding and E-selectin expression. (A) Effect of POVPC on PGPC action. HAEC were untreated (Control), coincubated for 4 h with PGPC alone (5 μg/ml) or PGPC (5 μg/ml) plus POVPC (5 μg/ml), and polymorphonuclear neutrophil (PMN) binding or E-selectin expression was measured. (B) Effect of POVPC and OxPAPC on LPS action. HAEC were coincubated for 4 h with LPS (2 ng/ml) and either POVPC at concentrations shown (μg/ml) or OxPAPC (100 μg/ml), and polymorphonuclear neutrophil binding and E-selectin expression was determined. Data are expressed as % reduction (mean ± SD), where 0% represents cells treated with LPS only and 100% represents cells treated with medium only. These data are representative of four experiments. ∗, P < 0.001 as calculated by one-way ANOVA, n = 6 fields or 6 wells/condition.