Figure 5.

Effect of POVPC and OxPAPC on LPS-induced E-selectin mRNA. (A) HAEC were incubated for 4 h with no additives (lane 1), LPS (2 ng/ml, lane 2), 5 μg/ml POVPC (lane 3), or LPS plus POVPC (lane 4), and Northern blotting was performed. 36B4 was used to normalize for RNA loading. (B) HAEC were incubated for 4 h with no additives (lane 1), LPS (2 ng/ml, lane 2), 100 μg/ml OxPAPC (lane 3), or LPS plus OxPAPC (lane 4), and Northern blotting was performed. 28S was used to normalize for RNA loading. (C) Nuclear runoff: HAEC were untreated, treated with LPS (2 ng/ml), treated with OxPAPC (100 μg/ml), or treated with LPS plus OxPAPC for 1 h. Nuclear runoff experiments were performed as described in Materials and Methods. ³²P-labeled RNA (1 × 10⁶ cpm) was used to probe a nylon blot prefixed with 2 μg of E-selectin or α-tubulin cDNA, as indicated.