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. 1999 Oct 12;96(21):12033–12038. doi: 10.1073/pnas.96.21.12033

Figure 2.

Figure 2

Efficiency in detecting EBV-reactive ex vivo-isolated CD8+ T lymphocytes at low concentrations of synthetic peptide antigens. In IFN-γ ELISPOT assay, CD8+ T cells (105 per well) freshly isolated from PBMC of healthy donor IP1 were seeded with autologous immature DC pulsed with a series of 1:10 dilutions of latent EBV peptide antigens LMP-2 329–337 (open bar), LMP-2A 426–434 (shaded bar) or EBNA-3A 379–387 (filled bar). Spots developed after a culture period of 20 h were evaluated and presented as described in Fig. 1. The data are representative experiment of two experiments performed.