Figure 5.

LMP-2A-specific CTL clone recognizes the naturally processed target epitope acid-eluted from LCL. A CTL clone recognizing the LMP-2A 426–434 epitope in association with HLA-A2.1 (15) was tested in a ⁵¹Cr-release assay at the indicated effector/target ratios for cytolytic activity against the HLA-A2.1-positive LCL derived from donor IP1 (♦) and against DC generated from IP1 and loaded with HPLC-fractionated (fraction 45, ■) or unfractionated (▴) naturally processed peptides acid-eluted from IP1’s LCL at a ratio of 10⁸ LCL cell equivalents of peptide per well. Control targets were untreated DC (□) and DC pulsed with 10⁻⁸ M of the synthetic LMP-2A 426–434 peptide epitope (○). Results were confirmed in two independent experiments.