Figure 2.

Vpr is packaged into virions. (a) Concentrated virus preparations were analyzed for the presence of Gag. Blots were probed with a human hyperimmune sera. The Gag species, p24 and p17, are indicated by arrows. (b) Concentrated virus preparations were subject to Western blot analysis and probed with an anti-Vpr antibody. pHR’Vpr produces wild-type Vpr and Vpr containing an N-terminal influenza hemagglutinin tag caused by the presence of a second initiation codon before Vpr. The protein doublet in the HR’Vpr lane is caused by the detection of both forms by the anti-Vpr sera. The arrow indicates wild-type Vpr.