Figure 5.

Uptake of labeled HDL by isolated hepatocytes. Hepatocytes were isolated from wt and apoE0 mice. Cells were incubated with doubly labeled HDL (0.5 μg/ml) for 1 h at 37°C. (A) Specific uptake from apoE0 HDL by wt and apoE0 hepatocytes. (B) Specific uptake from wt HDL by wt and apoE0 hepatocytes. Selective uptake represents the difference (³H − ¹²⁵I). Data were contained in four independent experiments in A and three in B, respectively. There are significant differences among apoE0 HDL/apoE0 hepatocyte vs. apoE0 HDL/wt hepatocyte (P < 0.05), wt HDL/apoE0 hepatocyte vs. apoE0 HDL/wt hepatocyte (P < 0.01), apoE0 HDL/apoE0 hepatocyte vs. wt HDL/wt hepatocyte (P < 0.05), and wt HDL/wt hepatocyte vs. wt HDL/apoE0 hepatocyte (P < 0.05). Inset shows the effect of human apoE cDNA transfection on selective uptake. Primary cultures of hepatocytes were transfected with human apoE3 expression plasmid or a mock plasmid by using Effectene (Qiagen). Uptake was measured after 4 h at 37°C.