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. 1999 Oct 12;96(21):12056–12061. doi: 10.1073/pnas.96.21.12056

Figure 3.

Figure 3

Analysis of genome and sg mRNA synthesis by leader and body TRS mutants. At 12 h posttransfection, total i.c. RNA was isolated, separated in denaturing 1.5% agarose gels, and hybridized (18) to a radiolabeled oligonucleotide probe complementary to the 3′ end of the genome. (a) Analysis of constructs containing single or double C-to-G substitutions in leader TRS (L series), RNA7 body TRS (B series), or both TRSs (LB series). (b) Analysis of a similar set of mutants in which the entire TRS has been mutated (5′-UCAAC-3′ to 5′-AGUUG-3′).