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Immunocytochemical detection of the β3 subunit in the neuropil in the hippocampus. (A) Experiment is performed with the purified anti-β3 antibody. (B) Experiment is performed with the preimmune serum of the rabbit that produced the anti-β3 antibody afterward. Note that the cell nuclei are nonspecifically labeled (particularly in the pyramidal cell layer), as demonstrated by the labeling also present with the preimmune serum.
