Figure 3.

The Golgi cell–granule cell GABAergic synapse is immature in waggler mice. (A) Spontaneous GABA_A responses with kynurenic acid (2 mM) in the bath solution. Each sweep represents a 1-s period, and the scale bar applies to all three traces. Response kinetics in adult waggler (P35) and young wild-type (P17) mice are slower than that of adult wild-type (P35). (B) Scaled spontaneous GABA_A responses. Each trace is an average of 20 events. (C) Relative amplitude (in percent) and decay time constant of the two exponentials fitted to the decaying phase of the GABA_A response. Each trace used in the fitting was an average of all events during a 1-min recording period. (D) In situ hybridization detected a 20% reduction of GABA_A receptor α6 subunit mRNA level in adult waggler cerebellum. Shown here are typical examples.