Table 1.

Induction of CFU-erythroid colonies by EPO and compound 5

Treatment	Dose	Avg. no. colonies per treatment
		Erythroid colonies	GM colonies
None	—	0  ±  0	86  ±  13
DMSO	0.4%	0  ±  0	74  ±  2
EPO	100 mU/ml	66  ±  1	87  ±  7
DMSO + EPO	100 mU/ml	55  ±  2	73  ±  5
	30 mU/ml	48  ±  3	73  ±  5
	10 mU/ml	20  ±  7	72  ±  13
	3 mU/ml	2  ±  1	65  ±  5
Compound 2	2000 nM	0  ±  0	65  ±  15
	500 nM	0  ±  0	66  ±  11
	125 nM	0  ±  0	72  ±  11
Compound 5	2000 nM	47  ±  2	65  ±  1
	1000 nM	43  ±  1	76  ±  1
	500 nM	30  ±  2	75  ±  5
	250 nM	12  ±  2	71  ±  1
	125 nM	3  ±  1	75  ±  1

Human CD34⁺ progenitor cells (1.5 × 10³/plate) were plated in semisolid media to allow growth of erythroid colonies and GM colonies. Colonies of each type were counted after 12–15 days of seeding under each condition. Data are mean (SEM) of two independent experiments, where each determination was performed in triplicate.