Figure 1.

Diazoxide activates SUR2A/Kir6.2 K_ATP channels in the presence of ADP. (A) Representative current trace recorded at −50 mV from an inside-out patch excised from an oocyte expressing Kir6.2 and SUR1. (B) Idem with SUR2A. (C) Average effects on SUR1/Kir6.2 and SUR2A/Kir6.2 currents of, from left to right, the vehicle DMSO, diazoxide in the absence of ADP, ADP, and diazoxide in the presence of ADP. For the latter, control represents the current measured in the presence of ADP. Numbers above bars indicate the number of patches tested. Responses were recorded as in A and B in the presence of 100 μM ATP and 1 mM Mg²⁺ by using 100 μM ADP, 300 μM diazoxide, and 0.3% DMSO.