Coleoptile bending and redistribution of endogenous IAA concentration in response to gravistimulation. (A Left) Time-dependent coleoptile bending (0–240 min) in response to a 90° gravistimulation. (Right) Cartoon of a gravistimulated coleoptile. (B) IAA concentration (pg IAA/mg fresh weight, mean of n = 3 experiments) in 0.5-cm segments of coleoptile halves, gravistimulated for 0, 5, 10, 15, 30, 45, and 60 min. The IAA spectrum was decomposed into seven concentration ranges and highlighted by a color code. 1, 10.8–13.9 ± 3.0; 2, 14.1–17.9 ± 3.3; 3, 19.9–23.5 ± 5.5; 4, 16.9–31.2 ± 5.9; 5, 34.6–42.5 ± 7.6; 6, 48.7–49.4 ± 6.5; 7, 51.1–58.8 ± 8.0 pg IAA/mg fresh weight.