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. 1999 Oct 12;96(21):12192–12197. doi: 10.1073/pnas.96.21.12192

Figure 1.

Figure 1

The effects of PtdOH and buOH on stomatal aperture. (A) Epidermal peels were kept in the light for 1 hr to induce stomatal opening and then treated (still in the light) as indicated. (B) Bright-field images of stomata treated as described. (Bar = 10 μm.) (C) Epidermal peels were kept in the dark for 1 hr to induce stomatal closure and then transferred to the light and treated as shown. Concentrations used: (+/−)-ABA, 15 μM; PtdOH, LysoPtdOH, DAG, and PtdBut, 50 μM; 1- and 3-buOH, 0.1% (vol/vol). (D and E) Epidermal peels were prepared as for A and treated with a range of PtdOH concentrations (D) or with ABA plus a range of 1-buOH concentrations (E). Stomatal apertures were measured after 1 hr of treatment and compared with the aperture of untreated controls [light, 7.9 ± 0.2 μm (A); dark, 3.5 ± 0.3 μm (C)]. The results show the mean ± SEM of at least three independent experiments measuring at least 60 stomata on 3 separate peels.