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The effect of ABA on in vivo levels of PtdOH and DAG. (A and B) Fluorescence images of TLC plates separating PtdOH (A) or DAG (B), extracted from protoplasts prelabeled with the fluorescent PLD substrate NBD-PtdCho, and treated with 10 μM (+/−)-ABA. (C and D) Quantification of PtdOH and DAG levels from TLC plates as shown in A and B. The data shown in C and D are the mean ± SEM of at least three separate experiments for each time point.
