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The effect of ABA on in vivo levels of PtdOH, PtdBut, and PLD activity. Protoplasts and NBD-labeled phospholipids were prepared as for Fig. 4. (A and B) Quantification of NBD-PtdOH (A) and NBD-PtdBut (B) levels from protoplasts treated with 10 μM (+/−)-ABA and 0.1% (vol/vol) 1-buOH. (C) PLD activity in extracts of protoplasts. The data show the mean ± SEM of at least three separate experiments for each time point.
