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. 1998 Jan 20;95(2):460–465. doi: 10.1073/pnas.95.2.460

Figure 3.

Figure 3

Northern blot analysis showing aminoacyl-tRNA and uncharged tRNA of representative mutants. tRNA genes with the indicated mutations were expressed from plasmid pGFIB in XAC/A16 cells. Samples containing ≈0.05 OD260 units of total tRNA isolated at pH 5.2 were fractionated by 6.5% PAGE in sodium acetate and urea. Percentage glutaminyl-tRNA is the average of several determinations (Table 1). We verified the glutaminyl-tRNA species by observing its signal increase from 22 to 51% when GlnRS was overproduced from plasmid pAC1.