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Western blot analysis of GlnRS from cells expressing representative mutant tRNAs. Serial dilutions of crude extracts were fractionated by 6% SDS/PAGE. “None” means XAC/A16 cells carrying plasmid pGFIB without an amber-suppressor tRNA gene. The position of purified GlnRS marker is noted. Cells overproducing GlnRS from plasmid pAC1 showed a 4- to 8-fold increase in GlnRS signal.
