Figure 1.

Interaction of GroEL with IMVs. (A and B) Cosedimentation of GroEL with IMVs after incubation at 37°C in the presence of 10 mM MgCl₂ (lanes 1–3) or 20 mM EDTA (lane 4). (A) Coomassie blue R-250 staining. (B) Western blotting using GroEL IgG antibodies. Lanes: 1, GroEL alone; 2, IMVs alone; 3 and 4, GroEL plus IMVs. (C) Heating of IMVs at 45°C inhibits irreversibly their subsequent interaction with GroEL at 30°C (Coomassie staining). Lanes: 1, GroEL alone, incubation for 30 min at 45°C; 2, IMVs alone, incubation for 30 min at 30°C; 3, GroEL plus IMVs, incubation for 30 min at 30°C; 4, GroEL plus IMVs, incubation for 30 min at 45°C and then for 30 min at 30°C; 5, IMVs alone, incubation for 30 min at 45°C and, after cooling to 30°C, GroEL was added and incubation was continued for 30 min at 30°C; 6, GroEL used as a marker.