Table 1.

Effect of adenine nucleotides and GroES on GroEL binding to IMV at 37°C

Experiment	Additions	GroEL binding, %
1	—	100
2	0.1 mM ADP	99  (66)
3	0.1 mM ADP + GroES	12
4	0.1 mM ATP	101  (23)
5	0.1 mM ATP*	30
6	0.1 mM ATP + GroES	15
7	0.1 mM ATP* + GroES	5

Molar ratio, GroES:GroEL = 2.5. Asterisk shows that the ATP-regenerating system (10 mM creatine phosphate and 50 μg/ml creatine kinase) was included. The numbers in parantheses indicate the GroEL binding to IMVs at 3.0 mM ADP or ATP.