Figure 3.
The dose-dependent effect of rMaspin(i) on plasminogen activation by free sctPA activity (A) and by poly-d-lysine-coimmobilized sctPA (B). The initial velocities are presented as the rate of plasmin production in units of pM/min (⧫). The error bars represent the standard deviations of two parallel experiments. The dashed line represents calculated initial velocities by using Eq. 2 with α = 0.01 pM/min, β = 8.3 × 10−7/min, ξ = 0.425, ψ = 1.7 × 10−7 pM−1, and ζ = 1.0 × 10−16 pM−2 for A and α = 0.435 pM/min, ξ = 0.1, ψ = 4.0 × 10−7 pM−1, and ζ = 1.0 × 10−16 pM−2 for B.