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Age-related differences in the effect of ischemia and reperfusion on ADP-dependent mitochondrial respiration. After perfusion (P), ischemia (I), or reperfusion (R) of hearts from 8- and 24-month-old rats, mitochondria were isolated and respiratory activities were assessed by using a Clark-style oxygen electrode. Mitochondria at a concentration of 0.5 mg/ml were incubated with 15 mM glutamate for 2.0 min at 25°C. State 3 respiration was then initiated by addition of 0.5 mM ADP.
