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. 1998 Jan 20;95(2):510–514. doi: 10.1073/pnas.95.2.510

Figure 2.

Figure 2

SDS/gel electrophoresis and Western blot analysis of protein from cardiac mitochondria. Hearts obtained from 8- and 24-month-old rats were exposed to perfusion (P), ischemia (I), or reperfusion (R). Mitochondria were then isolated and proteins were resolved by SDS/gel electrophoresis using duplicate 10% gels (12.5 μg of total protein per lane). One gel of the series was transferred to a nitrocellulose membrane for Western blot analysis with anti-HNE antibody (A). The second gel was stained with Coomassie blue for analysis of the molecular weight distribution of mitochondrial protein (B).