Table 1.

Mitochondrial yield and respiratory activity

Protocol	Yield, mg/g of heart	State 3, nmol of O per min per mg	State 4, nmol of O per min per mg	RCR State 3/state 4	ADP/O	Uncoupled, nmol of O per min per mg
8 month old
Perfused (n = 8)	8.8  ±  0.8	191.7  ±  18.3*	26.6  ±  4.5	7.5  ±  1.3	3.1  ±  0.1	186.8  ±  24.3**
Ischemic (n = 6)	8.6  ±  0.9	143.0  ±  17.9*	28.6  ±  0.8	5.0  ±  0.7	3.2  ±  0.3	151.2  ±  25.7**
Reperfused (n = 7)	8.4  ±  1.3	153.7  ±  20.8§	33.8  ±  4.4	4.6  ±  0.7	2.9  ±  0.2	149.0  ±  24.8§§
24 month old
Perfused (n = 5)	8.6  ±  0.8	191.9  ±  12.6†	30.2  ±  3.2	6.4  ±  0.4	2.9  ±  0.2	190.2  ±  12.5‡‡
Ischemic (n = 5)	8.2  ±  1.1	149.0  ±  15.4†‡	31.2  ±  4.2	4.8  ±  0.6	2.9  ±  0.1	156.7  ±  11.4‡‡††
Reperfused (n = 8)	8.8  ±  1.6	105.4  ±  8.9§‡	27.0  ±  3.9	4.0  ±  0.4	2.8  ±  0.2	94.7  ±  15.8§§††

Values are the mean ± SD. RCR, respiratory control ratio. P values (determined from paired t test) where like symbols indicate values compared. State 3: *, ≤0.001;  

^†, ≤0.004;  

^‡, ≤0.003;  

^§, ≤ 0.001. Uncoupled: **, ≤0.03;  

^‡‡, ≤0.008;  

^††, ≤0.0001;  

^§§, ≤0.001. Comparison of state 3 values with those of uncoupled respiration at a given experimental protocol (i.e., 8-month-old perfused state 3 vs. perfused uncoupled) indicated that differences were not statistically significant.