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. 1998 Jan 20;95(2):525–530. doi: 10.1073/pnas.95.2.525

Figure 2.

Figure 2

DNA binding of Ku, p460, and the Ku–p460 complex. (A) DNA binds to Ku, p460, and Ku–p460 complexes in distinct complexes that are affected differently by poly(dT). Labeled f32 DNA (0.2 ng) and various amounts of poly(dT) were incubated with Ku (11 ng), p460 (35 ng), or an equimolar mixture of both in 5 μl of buffer B containing 10 mM NaCl and analyzed by EMSA. Positions of individual complexes are indicated to the left of the gel. (B) Absence of Ku binding activity in the p460 preparation. Labeled f32 DNA (0.2 ng) with or without poly(dT) was incubated with Ku, p460, or a mixture of Ku and p460 (in a molar ratio of 1:100) in 5 μl of buffer B containing 10 mM NaCl and analyzed by EMSA. Gels were exposed to x-ray film for the indicated times.