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. 1998 Jan 20;95(2):525–530. doi: 10.1073/pnas.95.2.525

Figure 5.

Figure 5

DNA binding and kinase activation of p460 by f22 DNA are inhibited by the addition of Ku. (A) DNA binding. Labeled f22 DNA (1.4 ng, 100 fmol) was incubated with p460 (35 ng, 76 fmol) plus different amounts of Ku in 5 μl of buffer B containing 10 mM NaCl and then analyzed by EMSA. (B) Kinase activation. Kinase activity was measured for p460 (76 fmol) with different amounts of Ku in 5 μl of buffer C containing 10 mM NaCl, after stimulation by f22 DNA (100 fmol) or DpnI-cleaved plasmid DNA (13 ng, corresponding to 100 fmol of DNA fragments).