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Two-dimensional nonequilibrium pH-gradient gel electrophoresis–SDS/PAGE separation of protein extracts from D. immitis adult female worm. Silver-stained gel (Upper) and two-dimensional immunoblot (Lower) probed with rabbit antibodies to B. malayi pTGase show the 56-kDa protein spots (arrow). The two immunoreactive spots (Lower) had identical N-terminal sequences, and one of the spots (arrow) was used to determine the internal amino acid sequence of the protein.
