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. 1998 Jan 20;95(2):531–536. doi: 10.1073/pnas.95.2.531

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Copyright © 1998, The National Academy of Sciences

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Identification of native DiTG in the developmental stages of D. immitis. Immunoblot analysis was carried out with IgG-enriched anti-rDiTG antiserum and the following parasite extracts. Lanes: 1, 0-hr L₃ (200); 2, 48-hr L₃ (200); 3, 6-day L₄ (200); 4, larval E-S products (10 μg); 5, adult female worm (10 μg); 6, adult male worm (10 μg); 7, adult E-S products (10 μg); 8, rDiTG (100 ng, ).

Inline graphic — Identification of native DiTG in the developmental stages of D. immitis. Immunoblot analysis was carried out with IgG-enriched anti-rDiTG antiserum and the following parasite extracts. Lanes: 1, 0-hr L₃ (200); 2, 48-hr L₃ (200); 3, 6-day L₄ (200); 4, larval E-S products (10 μg); 5, adult female worm (10 μg); 6, adult male worm (10 μg); 7, adult E-S products (10 μg); 8, rDiTG (100 ng, ).