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In vitro cleavage of Bcl-xL by caspases. (A-C) The indicated in vitro-translated 35S-labeled proteins were incubated with the indicated recombinant caspases and analyzed by SDS/PAGE and fluorography. The D61A mutation causes an altered migration in SDS/PAGE (see Discussion). Met45 of human Bcl-xL (an Arg in rat Bcl-xL) is presumed to give rise to an internal initiation product marked by an asterisk throughout.
