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. 1998 Jan 20;95(2):554–559. doi: 10.1073/pnas.95.2.554

Figure 2.

Figure 2

Cleavage of Bcl-xL by apoptotic cell extracts and in cells induced to undergo apoptotic cell death. (A) An in vitro translation mix containing 35S-labeled wt or mutant Bcl-xL protein was incubated with recombinant caspase-1, apoptotic cell extract (S/M), or control extract (IP) with or without inhibitors and analyzed by SDS/PAGE and fluorography. Induction of apoptotic morphology in HeLa cell nuclei by the S/M extract and lack of apoptosis with the IP extract was confirmed by 4′,6-diamidino-2-phenylindole staining (data not shown). (B) An independent experiment in which cleavage of in vitro-translated Bcl-xL by the S/M extract was inhibited by Ac-DEVD-CHO. ∗, See legend to Fig. 1. (C) Cleavage of endogenous Bcl-xL in COS-1 cells after Sindbis virus infection. Virus-infected or ΔN61-transfected cell lysates were immunoblotted with 2A1 antibody.