Figure 4.
The caspase cleavage site in Bcl-xL is important for anti-death activity in the context of a BH1 mutation. (A and B) BHK cells were mock-infected or infected with recombinant Sindbis viruses expressing wt Bcl-xL or the indicated Bcl-xL mutants. The results are the mean ± SE of three to four independent experiments, counting at least 500 cells per sample. (A) An immunoblot of similarly infected cell lysates. The antibody does not detect the loop deletion mutant. Control recombinant viruses expressing Bcl-xL in the reverse orientation are indicated as “rev.”