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Western blot analysis of embryos injected with RNA encoding normal and mutant forms of XLerk. 2-Cell embryos were injected with 2.5 ng of RNA encoding all XLerk forms, except in the cases of XLerk215–327,215–307,215–287, where 0.5 ng of RNA were used. Extracts were prepared from stage 9 embryos, and three embryo equivalents were fractionated on 12% SDS-PAGE and then immunoblotted for the Flag epitope.
