Figure 5.

(A) Western analysis of embryos overexpressing XLerk and C-cadherin. Embryos were either left alone or injected with 4 ng of XLerk RNA, or coinjected with 1 ng of C-cadherin RNA. At stage 9, extracts were prepared, and three embryo equivalents were fractionated by SDS-PAGE. Samples were immunoblotted with C-cadherin antibodies and a C-terminal XLerk antibody (cadherin appears as a doublet, perhaps representing the precursor and product forms). (B) C-Cadherin and β-catenin expression and association in XLerk-injected embryos. Embryos were either left alone or injected with 5 ng of XLerk RNA. Stage 9 embryos were harvested, and extracts were prepared. Three embryo equivalents were fractionated by SDS PAGE, and five embryo equivalents were subjected to immunoprecipitation analysis using β-catenin antibodies (β-Cat IP) and then fractionated by SDS-PAGE. Samples were immunoblotted with antibodies to β-catenin, C-cadherin, or phosphotyrosine.