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. 1998 Jan 20;95(2):594–599. doi: 10.1073/pnas.95.2.594

Figure 2.

Figure 2

Expression of steroidogenic protein mRNAs in the testes of transgenic animals. (Upper) Northern blot hybridization of StAR (Left) and P450scc, 3β-HSD, and P450c17 probes (Right) to testicular RNA extracted from 1) normal littermate of MT-hAMH transgenic, 2) MT-hAMH transgenic, 3) adrenals of normal mice, 4) normal littermate of AMH-deficient transgenic, and 5) AMH-deficient mouse. Results shown are representative of those obtained for nine MT-hAMH and five AMH-deficient animals and the same number of normal littermates. Total RNA samples were 20 μg for testicular tissue and 5 μg for adrenal; exposure was 4 hr for P450scc, 12 hr for 3β-HSD and P450c17, and 48 hr for StAR. (Lower) PhosphorImaging quantification of steroidogenic proteins mRNA expression in MT-hAMH and AMH-deficient mice. Results are expressed as mean ± SEM (n = 9 for MT-hAMH and n = 5 for AMH-deficient mice) of percentage of the expression relative to a normal littermate (control) analyzed on the same blot. ∗, P ≤ 0.05; ∗∗, P ≤ 0.01; ∗∗∗, P ≤ 0.001.