Table 5.
ChiLl stimulates RexAB-mediated recombination
| E. coli strain (plasmids) | Transformation efficiency
|
No. Ampr transformants* (No. tfo)
|
ChiLl stimulation† | |
|---|---|---|---|---|
| pACYC184 (1 μg) | ChiLl‡ | ChiEc‡ | ||
| recB21C22 | 3 × 109 | 650 | 22 | 27† |
| (pΔB1a, pRexAB) | (7) | (7) | ||
| recB21C22 | 8 × 109 | 8 | 7 | 1 |
| (pΔB1a) | (2) | (2) | ||
| Wt | 1.5 × 1010 | 615 | 567 | 1† |
| (pGB2:PAragam, pΔB1a) | (4) | (4) | ||
*
Values represent total number of transformants from multiple experiments (number in parentheses).
†
ChiLl stimulation is the ratio of Ampr transformants obtained with linear DNA fragments containing ChiLl compared with ChiEc. The presented ratio was corrected by taking into account transformation efficiencies in the strain depleted for Chi activity (TG1 containing pGB2:PAragam and pΔB1a).
‡
ChiLl and ChiEc correspond to double Chi sites present at ends of linear fragments used for gene conversion.