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Selection of mutants. Primary selection of mutant candidates: hygromycin-resistant seedling after 8 days selection on 15 mg/l hygromycin (a), Northern (b), and Southern (c) blot analysis of the parental line A with the silent hpt gene and the mutants som1-som8. Both membranes were probed with the hpt coding region. Genomic DNA was digested with BamHI.
