Table 1.
Isolation and characterization of som mutants
| Mutant
|
||||||||
|---|---|---|---|---|---|---|---|---|
| som1 | som2 | som3 | som4 | som5 | som6 | som7 | som8 | |
| Mutagen | EMS | EMS | EMS | FNR | FNR | FNR | FNR | FNR |
| Hygromycin resistance | + | + | + | + | + | + | + | + |
| Transcriptional activity | + | + | + | + | + | + | + | + |
| Integrity of transgenic insert | + | + | + | + | + | + | + | + |
| Other candidates in the same M2 family | 0 | 0 | 2 | 4 | 4 | 0 | 3 | 5 |
| Self-fertility | + | + | − | + | + | + | + | + |
| Transgene genotype | Hemizygous | Hemizygous | Homozygous | Homozygous | Homozygous | Homozygous | Hemizygous | Hemizygous |
| Resistance in selfed progeny | + | + | + | + | + | + | + | |
| Number of seedlings tested | 117 | 116 | 67 | 29 | 26 | 67 | 60 | |
| Number of resistant seedlings | 50 | 69 | 59 | 25 | 18 | 40 | 35 | |
| Percentage of resistance | 43% | 60% | 88% | 86% | 69% | 60% | 58% | |
| Trans-activation of silent locus* | + | + | + | + | + | + | + | + |
| Number of seedlings tested | 373 | 626 | 175 | 133 | 126 | 141 | 289 | 125 |
| Number of resistant seedlings | 45 | 98 | 27 | 26 | 18 | 32 | 45 | 19 |
| Percentage of resistance | 12.1% | 15.7% | 15.4% | 19.5% | 14.3% | 22.7% | 15.6% | 15.2% |
*Compare with Fig. 2.