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. Author manuscript; available in PMC: 2007 Apr 2.
Published in final edited form as: Front Biosci. 2006 Sep 1;11:2230–2248. doi: 10.2741/1966

Figure 10.

Figure 10

UVB radiation stimulates CRH in human adult dermal fibroblasts. Fibroblasts were stimulated with UVB radiation, and after 24 hours RNA was extracted from cells and protein collected. A: CRH mRNA levels were measured with real-time RT-PCR based on Taqman chemistry (Applied Biosystems, assay Hs00174941_m1 for CRH and Hs99999901_s1 for 18SrRNA). Results are presented as mean ± SEM (n=3), **p<0.005. A, insert: CRH level was measured with ELISA (Phoenix Pharmaceuticals, Belmont, CA). Results are presented as mean ± SEM (n=3), *p<0.05. B: POMC mRNA levels were measured with the real-time RT-PCR based on Sybr Green chemistry (37). Bands representative of products of POMC and 18SrRNA PCR reactions are shown in the insert.