Skip to main content
. Author manuscript; available in PMC: 2007 Apr 2.
Published in final edited form as: Front Biosci. 2006 Sep 1;11:2230–2248. doi: 10.2741/1966

Figure 4.

Figure 4

Corticotropin-releasing hormone (CRH) increases CREB binding activity in HaCaT cells. HaCaT cells were stimulated with 100 nM CRH in serum free Dulbecco’s modified Eagles’s medium (DMEM) for 0 (control), 15, 30 and 60 minutes. Nuclear extracts were subjected to electrophoretic mobility shift assay analysis using a 32P-labelled CREB oligonucleotide probe (5′-AGA GAT TGC CTG ACG TCA GAG AGC TAG -3′) as described (42, 53). Cold represents nuclear extract pre-incubated with 50X excess of cold oligonucleotide.