Figure 2.
Sequence alignment of the conserved J domains of E. coli DnaJ and the S. cerevisiae DnaJ proteins with J domains located in the lumen of the ER. The multiple alignments were performed with ClustalX [51]. Numbers in the left and the right indicate first and last amino acids of each aligned sequence. The four α-helical regions of the J domain of E. coli DnaJ and its human homologue HDJ1 that were determined by NMR structure [37] are indicated. The highly conserved HPD tripeptide, a hallmark of the J domains that mediate interactions with Hsp70s, and essential amino acids identified in E.coli J domain are in black boxes.