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. Author manuscript; available in PMC: 2008 Feb 1.
Published in final edited form as: Biochim Biophys Acta. 2006 Oct 26;1773(2):232–242. doi: 10.1016/j.bbamcr.2006.10.011

Figure 6.

Figure 6

Northern blot analysis. Yeast cells RNA preparation and Northern blot analysis were performed as described in Materials and Methods. A) Induction of the ERJ5 mRNA by agents that promote the accumulation of unfolded proteins in the ER. A wild type strain (RGY132) was grown at 24ºC in the absence or presence of 10 μg/ml of tunicamycin (Tm) or 10 mM DTT. ERJ5 mRNA, normalized to ACT1 mRNA probed on the same blot, is expressed as bars corresponding to the fold-induction of ERJ5 mRNA relative to the expression in the wild-type in absence of the treatments. B) Induction of the unfolded protein response. KAR2 mRNA normalized to ACT1 mRNA probed on the same blots, is expressed as bars corresponding to the fold-induction of KAR2 mRNA relative to the expression in the wild-type in absence of treatments (left and center panels), or as fold-induction relative to the Δscj1Δjem1 strain (right panel). Representative Northern blots of several independent repetitions are shown.