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. Author manuscript; available in PMC: 2007 Apr 2.
Published in final edited form as: J Mol Recognit. 2007;20(1):39–50. doi: 10.1002/jmr.809

Figure 7.

Figure 7

Affinity precipitation of cIAP1 and XIAP from cell lysates with the E92 truncation (AVPWGGKGLAA), the F42 (SRVWKAPQYSDEFV) peptide, and the W → A F42 mutant (SRVAKAPQYSDEFV). Approximately 250 μg of total protein from a 293 cell lysate were incubated with 1.2 μg of the indicated peptides immobilized on streptavidin-coated beads. The bound proteins were resolved by SDS–PAGE and immunoblotted with a cIAP1 monoclonal antibody. The same blot was re-probed with a XIAP monoclonal antibody. The input lane contains 10% of the lysate protein used in the pull-down experiments.