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Histograms displaying the three hour cumulative shear-induced nitric oxide production after manipulation of a specific component of the endothelial glycocalyx with: heparinase (Hep.), neuraminidase (Neur.), chondroitinase (Chond.) or hyaluronidase (Hyal.). The boxed values at the top represent the level of reduction of each glycocalyx component, deduced from fluorescent imaging or ELISA assay (hyaluronan). n is the number of replications; * denotes statistical significance (p<0.05).
