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. Author manuscript; available in PMC: 2007 Apr 2.
Published in final edited form as: Genomics. 2006 Oct 17;89(2):197–206. doi: 10.1016/j.ygeno.2006.09.006

Figure 2.

Figure 2

RT-PCR experiments confirming expression of predicted transcripts in the inner ear. Templates were mRNA derived from liver, kidney, pancreas, retina, brain, testes, inner ear and genomic DNA. Primers were designed to span at least one intron at the 3′ end of a transcript predicted by an MPSS signature. Control primers for the mouse beta-actin gene, Actb, amplify different size products from cDNA and genomic DNA templates, and are used in RT-PCR as a loading control. The RT-PCR products depicted here were cloned and sequenced, and were the templates for in situ RNA probes.

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