Figure 4.
Expression of Spry2 mRNA during murine embryonic lens morphogenesis. Sections of mouse eyes at embryonic stages E9.5 (A, E), E10.5 (B, F), E12.5 (C, G), E13.5 (D, H), E15.5 (I, J) and E18.5 (K, L) were labelled for Spry2 transcripts using fluorescence in situ hybridisation (E–H, J, L) and counterstained with Hoechst dye (A–D, I, K). Spry2 was ubiquitously expressed throughout lens morphogenesis, with strong expression in both the lens epithelium and primary fibre cells. Abbreviations: epi, lens epithelium; lp, lens pit; lv, lens vesicle ; oc, optic cup; ov, optic vesicle; pf, primary fibres. Scale bar: 50 μm (A, B, E, F); 100 μm (C, D, G, H); 80 μm (I–L).