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. Author manuscript; available in PMC: 2007 Nov 10.
Published in final edited form as: Biochem Biophys Res Commun. 2006 Sep 18;350(1):214–219. doi: 10.1016/j.bbrc.2006.09.032

Fig. 4.

Fig. 4

Both halves of hA3G are responsible for cytoplasmic retention. Constructs were designed that split hA3G in the predicted linker region between the two homologous halves of the protein. The C-terminal deletion hA3G mutant, a.a. 1-208 (A), N-terminal deletion hA3G mutant, a.a. 209-384 (C) and each mutant with an N-terminal SV40 NLS (B and D, respectively) localized to the cytoplasm when transfected into HeLa cells with or without (+/−) LMB treatment. The upper and lower panels for A–D are two representative fields of the same treatment group. DAPI staining indicates the position of the cell nuclei in each panel. The dashed lines indicate the missing portions of the deletion mutants.