Figure 1. Rat hepatic ACF immunoreactivity with anti-phosphoserine and threonine antibodies.
ACF was immunoprecipitated from liver nuclear extracts with anti-ACF CT antibody conjugated to Sepharose and western blotted with anti-phosphoserine, threonine, or tyrosine antibodies (right column). The blots were stripped and re-probed with anti-ACF NT antibody. ACF, position of ACF; anti-pSer, anti-pThr, anti-pTyr denotes position of phosphoserine, threonine, or tyrosine immunoreactivity.