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. Author manuscript; available in PMC: 2008 Mar 1.
Published in final edited form as: Biochim Biophys Acta. 2006 Dec 8;1773(3):408–418. doi: 10.1016/j.bbamcr.2006.11.019

Figure 3. PKC phosphorylates ACF64 in vitro.

Figure 3

Recombinant ACF64 was reacted with PKC isozymes (α βII, δ, ε and ζ) or PKA, transferred to nitrocellulose and analyzed by autoradiography. Western transfers were subsequently probed with anti-HA antibody. (−) denotes kinase-free control reaction only containing buffer and ACF.