Figure 3.

Quantitative comparison of MNase hypersensitivity. MNase hypersensitivity (expressed as percentage of total lane intensity) at ARG4 (A, C, and D) and CYS3 site I (B, E, and F) in mre2 (A), mre11 (B), mre11 rad50Δ (C), rad50Δ (D), xrs2 (E), and rad50S (F) is plotted as a function of MNase concentration. The mutant and wild-type cultures were always analyzed in parallel on the same day. For an accurate evaluation of the mutant phenotypes, all mutant data are indicated with wild-type data for an internal standard taken on the same day. Solid symbols, wild type; open symbols, mutants. Dashed lines, 0 h (premeiosis); solid lines, 4 h (meiosis). Note that the mutants are classified into groups (types 1, type 2, and rad50S, as indicated) and that the mutant effects are independent of the MNase concentration.