Fig. 3. UVB Stimulates Transcriptional Activity of CRH (A) and POMC (B) Promoters.
Normal human melanocytes were transfected with a reporter gene construct driven by the sequence of human CRH promoter (−969 to +7; p-CRH-Luc) or human POMC promoter (−771 to −8; p-POMC-Luc) and with PhRL-TK (transfection efficiency control). Cells were irradiated with UVB (0–100 mJ/cm2) and lysed at the time points indicated on the figure, when activity of the promoter was measured. Data are presented as mean ± SEM (n = 3).